Screening and mutagenesis of a novel Bacillus pumilus strain producing alkaline protease for dehairing

Enhanced Production of Alkaline Protease by a Mutant of Bacillus licheniformis N-2 for Dehairing

The purpose of the present investigations was to improve the yield of alkaline protease for leather dehairing by subjecting the indigenous proteolytic strain Bacillus licheniformis N-2 to various mutagenic treatments viz. UV irradiations, NTG (N-methyl-N-nitro-N-nitrosoguinidine) and MMS (methyl methane sulfonate). After screening on skim milk agar plates, a total of nine positive mutants were ...

Isolation and Identification of an Alkaline Protease Producing Bacillus from Soil

Isolation, Purification and Characterization of a Thermophilic Alkaline Protease from Bacillus subtilis BP-36

The goal of this research was to isolate and identify the thermostable alkaline protease producing bacteria among several native Iranian microorganisms. At the end of screening program, a Bacillus subtilis BP-36 strain producing thermophilic alkaline protease was isolated from a hot spring in Ardebil province. The target enzyme was purified using a one-step Aqueous two-phase systems (ATPS) prot...

Screening and Identification of a Novel Alkaline Lipase Producing Bacterium

The lipase producing bacteria were screened from the soil both qualitatively by Tween 80, rhodamine B agar assays and quantitatively by titrimetry with the objective of finding a bacterial strain capable of producing an alkaline lipase. The higher lipolytic activity of 17.43 U/ml was recorded for the isolate L13. Traditional methods viz. morphological, cultural and biochemical characteristics w...

Cloning and Enhanced Expression of an Extracellular Alkaline Protease from a Soil Isolate of Bacillus clausii in Bacillus subtilis

in the detergent industry. In this study, the extracellular alkaline serine protease gene, aprE, from Bacillusclausii was amplified by PCR and further cloned and expressed in B. subtilis WB600 using the pWB980 expression vector. Protease activity of the recombinant B. subtilis WB600 harboring the plasmid pWB980/aprEreached up to 1020 U/ml, approximately 3-folds higher than the nativ...